chanelink logo
CHANELINK
Türkçe

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

447
2024-01-23 14:07:18
Çeviriyi gör

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

İlgili öneriler

  • 3D printed chocolate: a delicious fusion of innovation and sustainable development

    In the era of sustainable development and cutting-edge technology, the integration of 3D printing and culinary art is not only an innovation, but also a proof of human creativity. Imagine in such a world, your desserts are not just coming out of the kitchen, but carefully designed and printed layer by layer. This is not a glimpse of the distant future, but the reality of today, as developers have ...

    2024-02-19
    Çeviriyi gör

  • Ortel launches advanced 1550nm laser to enhance LiDAR and optical sensing functions

    Ortel belongs to the Photonics Foundries group and has launched its latest innovative product - the 1786 1550 nm laser module, aimed at significantly improving optical sensing in various applications. This laser module is designed specifically for continuous wavelength operation and is a key component of systems that require coherent light sources for precise sensing in environments with fluctuati...

    2024-03-16
    Çeviriyi gör

  • Using laser welding technology to manufacture rotor shafts at the speed of light

    How can EMAG Laser Technology accelerate the production of critical powertrain components using its flagship product ELC 6 system?The rapid popularity of electric vehicles worldwide indicates that production planners must increase their efforts in producing key components of electric vehicles, particularly the rotor shaft. The importance of the rotor shaft as the core component for converting elec...

    2024-07-17
    Çeviriyi gör

  • Latest breakthrough! 3500W free output blue semiconductor laser

    The 3500W free output blue semiconductor laser beam is output in a free space manner, with a rectangular spot directly acting on the material surface without the need for fiber optics or laser processing heads. This laser has a wavelength of 455 ± 10nm, with continuously adjustable power and a maximum output power of over 3500W. It is mainly used for non-ferrous metal cladding, quenching, etc., to...

    2024-09-03
    Çeviriyi gör

  • LPKF 2024 H1 revenue up 15% year-on-year

    Recently, LPKF Laser, a leading supplier of innovative laser solutions in Germany, released its performance report for the first half of the 2024 fiscal year as of June 30, demonstrating the company's steady performance and forward-looking layout in a complex market environment. According to the financial report, LPKF Laser&Electronics SE achieved significant growth in comprehensive revenue ...

    2024-07-31
    Çeviriyi gör