chanelink logo
CHANELINK
Polski

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

784
2024-03-29 15:03:26
Zobacz tłumaczenie

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Powiązane rekomendacje

  • This semiconductor integrator launches laser chip and array technology

    Recently, Sivers Semiconductors, a well-known chip and integration module supplier in Sweden, announced that its subsidiary Sivers Photonics is partnering with ecosystem partners to showcase its advanced laser chip and array technology at the OFC conference in Santiago.The first on-site demonstration used Ayar Labs optical I/O and CW-WDM MSA compatible SuperNova ™ The light source is powered...

    2024-03-29
    Zobacz tłumaczenie

  • Innovating Photonics: Lithium Tantalate Provides Power for the Next Generation of Optoelectronic Circuits

    The new photonic integrated circuit technology based on lithium tantalate has improved cost efficiency and scalability, making significant progress in the fields of optical communication and computing.The rapid development of photonic integrated circuits (PICs) has revolutionized optical communication and computing systems, combining multiple optical devices and functions on a single chip.For deca...

    2024-05-14
    Zobacz tłumaczenie

  • Launching the world's strongest laser at a cost of 320 million euros

    Beijing, April 1st (Reporter Liu Xia) - The world's most powerful laser has been activated recently. On March 31st, the Physicist Organization Network reported that the system can enable laser pulses to reach a peak of 10 terawatts (1 terawatt=100 terawatts=1015 watts) within 1 femtosecond (1000 trillions of a second), which is expected to promote revolutionary progress in multiple fi...

    2024-04-03
    Zobacz tłumaczenie

  • Xi'an Institute of Optics and Fine Mechanics has made new progress in the research of intelligent optical microscopy imaging

    Recently, the State Key Laboratory of Transient Optics and Photonics Technology of Xi'an Institute of Optics and Fine Mechanics has made new progress in the research of intelligent optical microscopic imaging, and the research results were published online in the international high-level academic journal Opto Electronic Advances (IF: 15.3). The first author of the paper is Tian Xuan, a 2024 doctor...

    2024-09-09
    Zobacz tłumaczenie

  • The University of California has developed a pioneering chip that can simultaneously carry lasers and photonic waveguides

    A team of computer and electrical engineers at UC Santa Barbara, in collaboration with several colleagues at Caltech and another colleague at Anello Photonics, has developed a first-of-its-kind chip that can carry both laser and photonic waveguides. In a paper published in the journal Nature, the team describes how they made the chip and how it worked during testing.With the advent of integrated c...

    2023-08-10
    Zobacz tłumaczenie