chanelink logo
CHANELINK
Deutsch

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

447
2024-01-23 14:07:18
Übersetzung anzeigen

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Ähnliche Empfehlungen

  • Researchers have created the first organic semiconductor laser that can be operated without the need for a separate light source

    Researchers at the University of St. Andrews in Scotland have manufactured the first organic semiconductor laser to operate without the need for a separate light source - which has proven to be extremely challenging. The new all electric driven laser is more compact than previous devices and operates in the visible light region of the electromagnetic spectrum. Therefore, its developers stated that...

    2023-11-15
    Übersetzung anzeigen

  • TRUMPF high-power laser dynamic beam shaping technology creates opportunities for the electric vehicle industry

    It is reported that researchers from TRUMPF in Germany reported research on using dynamic beam shaping of high-power lasers to improve the productivity of hairpin stators, creating opportunities for the electric vehicle industry. Relevant research was published in "PhotonicsViews" under the title "Unlocking opportunities for the EV industry with beam shaping of high-power lasers".The electric vehi...

    2024-07-01
    Übersetzung anzeigen

  • AEROTECH releases updated AUTOMATION1 motion control platform

    Aerotech is a global leader in precision motion control and automation, and every release has made the Automation1 motion control platform even stronger and more user-friendly. Version 2.5 brings TCP socket interface (test version), Automation1 MachineApps HMI development, new auxiliary module for motor settings, and improved machine settings for galvanometer laser scanning heads.Automation1 conti...

    2023-08-14
    Übersetzung anzeigen

  • Researchers have manufactured chip based optical resonators that can operate in the ultraviolet (UV) and visible light regions of the spectrum

    Figure: Researchers have created a chip based ring resonator that operates in the ultraviolet and visible light ranges and exhibits record low UV loss. The resonator (small circle in the middle) is displayed as blue light.Researchers have created chip based photonic resonators that can operate in the ultraviolet (UV) and visible regions of the spectrum and exhibit record low UV loss. The ne...

    2023-10-06
    Übersetzung anzeigen

  • Laser communication is expected to completely change optical links

    Laser technology is becoming a game changer in the field of satellite communication (SATCOM), capable of creating ultra secure networks that can transmit large amounts of data at unprecedented speeds through satellite networks and constellations.With continuous progress, the industry is ready for growth and collaboration, seizing the untapped potential of disconnected populations. The ability to h...

    2023-09-20
    Übersetzung anzeigen