chanelink logo
CHANELINK
简体中文

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

974
2024-03-29 15:03:26
查看翻译

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

相关推荐

  • Researchers use liquid metal and laser ablation to create stretchable micro antennas

    Researchers have developed a new method of making micro stretchable antenna with water gel and liquid metal. These antennas can be used for wearable and flexible wireless electronic devices to provide links between devices and external systems for power transmission, data processing, and communication.Using our new manufacturing method, we have demonstrated that the length of liquid metal antennas...

    2023-09-19
    查看翻译

  • Improved spectrometer color filter array for software calibration without the need for laser

    Hackaday will launch cool projects that may stimulate others to expand and enhance it, and even move in a completely new direction. This is the way the most advanced technology continues to evolve. This DIY spectrometer project is a great example of this spirit. It comes from Michael Prathofer, who was inspired by Les Wright's PySpectrometer, a simple device pieced together by a pocket spectrom...

    2024-05-28
    查看翻译

  • Screen Innovation Launches Short Focus Elevated Electric Laser TV Projection Screen

    Screen Innovations has added a short focal lift electric screen solution to its component and material series, meeting the growing demand for large but hidden displays in small media rooms and company boards.Unlike traditional projection systems that require sufficient distance from the projector to the screen or perform best in a darkroom, pop-up laser TVs are only a few inches away from short fo...

    2023-10-27
    查看翻译

  • Amplitude launches femtosecond lasers for industrial applications

    Recently, French femtosecond pulse and high peak power (PW class) laser manufacturer Amplitude announced that the company has launched a newly designed Satsuma X femtosecond laser, setting a new benchmark for industrial environments.This product was first announced in 2022 and is now available in a brand new design with proven durability and versatility. In pursuit of excellence and customer satis...

    2024-07-02
    查看翻译

  • UK venture capital group acquires MicroLED developer Plessey

    Haylo Labs, a UK company recently established by former WaveOptics CEO David Hayes, has acquired microLED developer Plessey Semiconductors.Haylo says it also plans to invest more than £100 million scaling Plessey’s production capacity over the next five years at the firm’s GaN-on-silicon site in Plymouth and beyond, in anticipation of fast-growing demand for augmented and virtual reality (AR/VR) a...

    09-01
    查看翻译