chanelink logo
CHANELINK
简体中文

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

762
2024-01-23 14:07:18
查看翻译

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

相关推荐

  • Multi functional materials for solar cells and organic light-emitting diodes to achieve high performance and stability

    Through joint research, a team developed a 4-amino-TEMPO derivative with photocatalytic performance and successfully used it to produce high-performance and stable fiber like dye sensitized solar cells (FDSSCs) and fiber like organic light-emitting diodes (FOLEDs). This paper was published in the journal Materials and Energy Today.The developed 4-amino-TEMPO derivatives have the characteristic of ...

    2024-06-03
    查看翻译

  • Unlocking visible femtosecond fiber oscillators: progress in laser science

    The emergence of ultrafast laser pulses marks an important milestone in laser science, triggering astonishing progress in a wide range of disciplines such as industrial applications, energy technology, and life sciences. Among various laser platforms that have been developed, fiber optic femtosecond oscillators are highly praised for their compact design, excellent performance, and cost-effectiven...

    2024-03-28
    查看翻译

  • Omnitron Announces Partnership with Silex Microsystems to Mass Produce MEMS Scanning Mirrors for LiDAR

    According to reports, Omnitron Sensors, a pioneer in the development of MEMS sensing technology for large-scale and low-cost markets, recently announced that it will collaborate with Silex Microsystems, a subsidiary of Semielectronics, to mass produce MEMS scanning mirrors for LiDAR.Eric Aguilar, co-founder and CEO of Omnitron Sensors, said, "We have noticed a huge demand from manufacturers of adv...

    2023-09-19
    查看翻译

  • Processing application of ultrafast laser on bulk metallic glass

    Recently, an international research team led by Professor Zhang Peilei from the School of Materials Science and Engineering at Shanghai University of Engineering and Technology published a review paper titled "Research status of femtosecond lasers and nanosecond lasers processing on bulk metallic glasses (BMGs)" in the renowned journal Optics&Laser Technology in the field of optics and lasers....

    2023-09-18
    查看翻译

  • Infinira launches an optical solution for 1.6 Tbps ICE-D data centers

    Infinira, an expert in optical network solutions, announced the launch of a high-speed data center optical transmission module based on single-chip indium phosphide (InP) photonic integrated circuit (PIC) technology. The company claims that the module will connect at a speed of 1.6 terabits per second (Tb/s), while reducing the cost and power consumption per bit.Yingfeilang stated that its data ce...

    2024-03-18
    查看翻译