chanelink logo
CHANELINK
Nederlands

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

447
2024-01-23 14:07:18
Bekijk vertaling

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Gerelateerde aanbevelingen

  • This innovation will significantly improve the sensitivity of gravitational wave detectors

    In 2017, the detection of gravitational waves generated by the merger of binary neutron stars marked a significant breakthrough in physics. These waves reveal important information about the universe, from the origin of short gamma ray bursts to the formation of heavy elements.However, capturing gravitational waves from the merged residue remains a challenge as these waves avoid the detection rang...

    2024-04-17
    Bekijk vertaling

  • A major investment! Lumentum completes acquisition of research and development site in Carswell, UK

    Lumentum, a leading designer and manufacturer of innovative optical and photonic products, has announced that it has completed the acquisition of a site in Caswell, UK.Lumentum revealed that it has made significant investments in the site over the past two years and is currently undergoing development upgrades for its state-of-the-art cleanrooms and laboratories to continue to support the developm...

    2023-09-13
    Bekijk vertaling

  • New method doubles and accelerates thermal tuning of optical chips, supporting two current and voltage regulation methods

    Silicon based quantum chip technology is one of the hot research directions in the field of integrated photonics. Thanks to compatibility with CMOS technology and silicon material characteristics, silicon-based integrated optical chips and devices have many advantages such as low cost, small size, low power consumption, and high integration, providing an ideal platform for large-scale optical comp...

    2024-04-02
    Bekijk vertaling

  • The INRS camera captures transient events and is suitable for various scenarios such as high-speed LiDAR systems for autonomous driving

    It is reported that the National Institutes of Sciences (INRS) of Canada has developed a camera platform that can achieve cheaper ultra fast imaging through the use of ready-made components, which can be used in various applications.This new device aims to address some of the limitations of current high-speed imaging, including parallax errors and potential damage from pulse illumination. Th...

    2023-10-07
    Bekijk vertaling

  • The Glory of Laser and the Odyssey of "Deep Technology"

    The British engineering and construction company Metz Group has a delegation in Spain to be responsible for the expansion and renovation of the central laser facility at Rutherford Appleton Laboratory near Oxford. More commonly, the construction of the powerful laser Vulcan 20-20 has just been obtained, with a delivery date of 2029.It will emit a main excitation beam that is billions of times larg...

    2023-12-09
    Bekijk vertaling