chanelink logo
CHANELINK
한국어

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

214
2024-03-29 15:03:26
번역 보기

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

관련 추천

  • Bitsensing, a South Korean LiDAR solution provider, successfully raised 180 million yuan in funding

    Recently, Bitsensing, a leading provider of advanced radar solutions in South Korea, announced the successful completion of Series B financing, with a financing amount of up to $25 million (approximately RMB 181.6 million).This major investment is led by a series of well-known venture capital firms and strategic investors, which not only demonstrates Bitsensing's leading position in the radar tech...

    2024-06-27
    번역 보기

  • The Institute of Physics, Chinese Academy of Sciences has made significant progress in the research of lithium niobate nanooptics

    In recent years, breakthroughs in the preparation technology of lithium niobate single crystal thin films have greatly promoted the important application of lithium niobate crystals in micro nano optical devices such as optical metasurfaces. However, the high hardness and inactive chemical properties of lithium niobate crystals pose significant challenges to micro nano processing; In addition, con...

    04-15
    번역 보기

  • Laser link between European Space Agency containers and space

    The latest expansion of the European Space Agency's laboratory is essentially portable: this European Space Agency's mobile optical ground station is housed in a standard container and can be transported throughout Europe as needed for laser based optical communication with satellites - including NASA's Psyche mission, in space millions of kilometers away.The station has officially become a part o...

    2024-02-12
    번역 보기

  • Laser gyroscopes measure small changes in daytime length on Earth

    Recently, scientists used laser gyroscopes to measure that the change in Earth's rotational speed is less than one millionth. This technology can help scientists understand the complex flow of water and air, which can cause the smallest adjustments to the Earth's rotation.The Earth's rotation is not completely stable. Planets accelerate or slow down as they rotate, slightly shortening or prolongin...

    2023-09-19
    번역 보기

  • Brother launches a series of color LED laser printers for homes and offices

    Brother is an innovative global company that proudly launches its latest series of color LED laser printers. The new printer series is colorful and seamlessly connected, designed specifically for home and small office environments.The company's latest product aims to improve productivity in home and small business environments, combining excellent printing quality with excellent printing speed. Ea...

    2024-03-20
    번역 보기