chanelink logo
CHANELINK
Français

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

762
2024-01-23 14:07:18
Voir la traduction

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Recommandations associées

  • Scientists use glass to create femtosecond lasers

    Image source: Federal Institute of Technology in Lausanne, SwitzerlandScience and Technology Daily, Beijing, September 27th (Reporter Zhang Jiaxin) Commercial femtosecond lasers are manufactured by placing optical components and their mounting bases on a substrate, which requires strict alignment of optical components. So, is it possible to manufacture femtosecond lasers entirely from glas...

    2023-09-28
    Voir la traduction

  • Laser giant seeks $100 million financing for $422 million debt restructuring

    On August 6th local time, Luminar, a leading publicly traded company in the field of LiDAR, announced a $422 million debt restructuring and raised $100 million in new capital. This measure marks Luminar taking solid steps in optimizing its capital structure and enhancing its financial stability.In early May this year, this laser radar manufacturer released an open letter disclosing a major strateg...

    2024-08-09
    Voir la traduction

  • Shanghai Institute of Optics and Mechanics proposes a new scheme of Er doped silicate fiber as an extended L-band broadband amplifier

    Recently, Hu Lili, a research group of the Advanced Laser and Optoelectronic Functional Materials Department of the Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, proposed a new scheme based on field strength optimization of Er doped silicate fiber as an extended L-band broadband amplifier. Relevant research achievements were published in Optics Letters under the tit...

    2024-06-05
    Voir la traduction

  • NASA will demonstrate laser communications on the space station to improve space communications capabilities

    Recently, in order to improve the National Aeronautics and Space Administration (NASA) space communications capabilities, NASA plans to send a technology demonstration called "Integrated LCRD Low Earth Orbit User Modem and Amplifier Terminal (ILLUMA-T)" to the space station in 2023.ILLUMA-T and the Laser Communications Relay Demonstration (LCRD), launched in December 2021, will together comp...

    2023-09-04
    Voir la traduction

  • The birth of multi photon 3D laser printing technology: printing millions of particles within 1 second

    Multi photon 3D laser printing technology, as a disruptive micro manufacturing technology, is facing two major challenges: speed and material compatibility. However, the latest research has made breakthrough progress, successfully increasing printing speed tenfold while maintaining excellent detail accuracy.In this remarkable study, scientists abandoned the traditional single beam printing method ...

    2024-04-19
    Voir la traduction